Process for preparing microorganisms used for making phenyl acetyl carlinol (PAC)
US5079145A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 21, 1988 |
| Grant date | Jan 7, 1992 |
| Priority date | — |
| Expiry date | Oct 21, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/942
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Mutations are induced in a microorganism selected from the species Saccharomyces cerevisiae or from the species Candia flareri. The resulting mutants are cultured in the presence of a fermentation inhibitor, such as acetaldehyde, ephedrine or PAC-dione, to form colonies having resistance to the inhibitor. Cells from the colonies are isolated and tested for yield of phenyl acetyl carbinol (PAC) in a fermentation with benzaldehyde and pyruvate. Yeast cells from the colonies that produce elevated levels of PAC are selected for use in subsequent fermentations. PAC is useful as an intermediate in the preparation of 1-ephedrine and d-pseudoephedrine, two well-known medicinal chemicals.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.