Immobilization of glycocompounds and glycoconjugates
US5082929A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 8, 1990 |
| Grant date | Jan 21, 1992 |
| Priority date | — |
| Expiry date | Aug 8, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K17/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for attachment of glycocompounds and glycoconjugates to hydrazide gels, wherein a solution of glycocompound or glycoconjugate containing a suitable oxidizing agent is brought into contact with the hydrazide gel without prior removal of the oxidizing agent. Suitably, the solution is introduced into a cartridge containing the hydrazide gel. In accordance with a first embodiment, this method requires relatively short incubations of about 30 minutes for oxidation of the glycosaccharide moiety of the glycocompound or glycoconjugate, followed by about 15 minutes for binding the oxidized glycocompound or glycoconjugate to the hydrazide gel. Pursuant to another embodiment, incubation with the oxidizing agent is carried out simultaneously with bringing the solution into contact with the gel (e.g., within the cartridge). Synthetic polymer gels with terminal hydrazide groups are particularly suitable for use in this method, as these gels are especially resistant to oxidizing agents such as sodium periodate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.