Method for corrective calibration of a flow cytometry using a mixture of fluorescent microbeads and cells
US5084394A · kind A · utility
25Cited by
20References
8Claims
0Family size
Inventors
Key dates
| Filing date | Apr 17, 1990 |
| Grant date | Jan 28, 1992 |
| Priority date | — |
| Expiry date | Apr 17, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The use of a combination of calibrated microbead populations with one or more calibrated biological cell populations to correct calibration of a flow cytometer for size and fluorescence intensity determinations of biological cell samples. The use of calibrated biological cells permits correction for factors related to the instrument and calibration microbeads so long as the excitation and emission spectra of the calibration microbeads, the calibration cells and the cell samples are all the same, respectively.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.