Fluidized bed separation and recovery of proteins from fluids in a rotary column
US5087369A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 22, 1991 |
| Grant date | Feb 11, 1992 |
| Priority date | — |
| Expiry date | Jan 22, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/79
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for separating and recovering very small amounts of biologically active proteins (such as enzymes, antigens and antibodies) present in a various fluids, with a high recovery and on a large scale, in which a fluid containing a desired protein is introduced into a rotary column through orifices therein and brought into contact with a gel carrier capable of selectively adsorbing the desired protein, while the carrier is being gentle fluidized in a liquid by the rotary motion successively by wash water and eluting fluid, so that the column, after which the fluid is displaced, the carrier having the desired protein adsorbed thereon is washed and the desired protein is recovered therefrom by elution while the carrier fluidized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.