Acyl carrier protein-I/protein-A gene fusion, products and methods

US5087563A · kind A · utility

Assignee

• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• Phillip D. Beremand · College Station, US
• Daniel J. Guerra · Troy, US

Key dates

• Filing date: Mar 9, 1990
• Grant date: Feb 11, 1992
• Expiry date: Mar 9, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/705
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A gene encoding spinach acyl carrier protein-I-Gly.sup.76 (ACP-I) was fused to a gene encoding the F.sub.c -binding portion of staphylococcal protein A. The gene fusion can be expressed in E. coli thereby resulting in the production of an appropriately sized protein possessing ACP activity in acyl ACP synthetase reactions, plant MCT reactions, and plant fatty acid synthesis. This gene fusion, under the control of the .lambda.P.sub.R promoter, expresses high levels of a 42 kDa fusion protein in E. coli. This protein functions as a affinity moiety in purification methods for the isolation of enzymes involved in fatty acid synthetase, acyl ACP synthetase and malonyl-CoA:ACP tranacylase reactions.