DNA probe diffraction assay and reagents
US5089387A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 7, 1988 |
| Grant date | Feb 18, 1992 |
| Priority date | — |
| Expiry date | Jul 7, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/757
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The assay of the subject invention uses DNA sequences as probes in a nucleic acid hybridization diffraction assay, to detect specific DNA sequences in a sample. Diffraction assay methodologies are applied to determine the presence and amount of analyte. This invention involves a discovery in the areas of supporting surfaces for a biogrid or biograting which provide greatly reduced non-specific hybridization and binding. A preferred process of this invention involves manufacturing a biograting for use in a light diffraction assay, and comprises adhering a uniform layer of hybridizing reagent comprising a nucleotide sequence on a smooth, solid surface and exposing the surface to UV radiation through a shadow mask with a diffraction grating pattern of lines to selectively deactivate the hybridizing reagent, leaving a biological diffraction grating design of lines of active hybridizing reagent. The smooth, solid surface is preferably selected from the group consisting of polysilicon and single crystalline silicon surfaces. The diffraction hybridizing assay method of this invention for determining the presence or quantity of an analyte in an aqueous sample comprises contacting a nucleic…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.