Method for the preparation of fructose-containing oligosaccharide
US5089401A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 20, 1991 |
| Grant date | Feb 18, 1992 |
| Priority date | — |
| Expiry date | Mar 20, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/83
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An enzymatic method for the preparation of a fructose-containing oligosaccharide, in which a .beta.-fructofuranosidase obtained by culturing Arthrobacter sp. K-1 (FERM BP-3192) as an enzyme is reacted on sucrose, raffinose or stachyose as the donor in the presence of an aldose or ketose as the receptor. The enzyme is characterized by: PA0 (1) activity on sucrose for the transglycosidation of a fructosyl group to the receptor in the presence of a monosaccharide, sugar alcohol, alkyl alcohol, glycoside or oligosaccharide; PA0 (2) activity for the decomposition of sucrose, elrose, neokestose, xylsucrose, raffinose and stachyose with inactivity on a saccharide selected from the group consisting of 1-kestose, nistose, inulobiose and levan biose; PA0 (3) optimum pH of 6.5 to 6.8 at 40.degree. C. with stability at a pH of 5.5 to 10; PA0 (4) optimum temperature of 55.degree. C. at a pH of 6.5 exhibiting at least 70% of residual activity at 60.degree. C.; PA0 (5) susceptibility to inhibition by the ions of silver, mercury, zinc, copper and tin; PA0 (6) two molecular weights of 52,000.+-.2,500 and 58,000.+-.2,500; and PA0 (7) two isoelectric points of pH 4.3 and pH 4.6.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.