Methods of generating desired amino-terminal residues in proteins
US5093242A · kind A · utility
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8Claims
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Key dates
| Filing date | May 24, 1989 |
| Grant date | Mar 3, 1992 |
| Priority date | — |
| Expiry date | May 24, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/94
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo are described. The method can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The method is based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.
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