Rapidly cleavable substrate for HIV protease

US5093477A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Karin Molling · Berlin, DE
• Stephan Henke · Bad Soden am Taunus, DE
• Gerhard Breipohl · Scheinfeld, DE
• Wolfgang Konig · Hofheim, DE

Key dates

• Filing date: Dec 13, 1989
• Grant date: Mar 3, 1992
• Expiry date: Dec 13, 2009

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A rapidly cleavable substrate for HIV protease Hepta- to nona-peptides of the formula EQU A--B--C--D--E--Opr--F--G--H, in which A is absent or represents Val, Ile or Thr, B denotes Ser, Thr or Phe, D denotes Asp or Ala, E denotes Tyr or Phe, Opr denotes 5-oxaproline, F denotes Ile, Leu, Val, Thr or Gln, G denotes Val, Ile, Ser or Arg, and H is absent or represents Gln, Glu, Pro or Thr, as well as the salts thereof are used for locating and detecting HIV protease and for testing possible inhibitors of HIV protease. They are prepared by known methods of peptide chemistry, preferably by means of solid-phase synthesis.