Chemiluminescence assays using stabilized dioxetane derivatives
US5094939A · kind A · utility
19Cited by
1References
11Claims
0Family size
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Key dates
| Filing date | Jul 19, 1989 |
| Grant date | Mar 10, 1992 |
| Priority date | — |
| Expiry date | Jul 19, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A multi-pH chemiluminescence-based assay method comprising first-stage light production by the activation by cleavage of a phosphatase cleavable dioxetane derivative within a pH range optimal for enzyme catalysis, followed by the second-stage generation of increased light energy from the products of enzyme-catalyzed cleavage of the dioxetane derivative by the adjustment of the pH to a strongly alkaline pH optimal for the generation of light energy. The assay method is suitable for both solution phase and solid state assay formats. Light generation enhancers can be used further to increase the light yield.
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