Generation and selection of novel DNA-binding proteins and polypeptides
US5096815A · kind A · utility
292Cited by
16References
42Claims
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Key dates
| Filing date | Jan 6, 1989 |
| Grant date | Mar 17, 1992 |
| Priority date | — |
| Expiry date | Jan 6, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/10322
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding protein and selection for proteins binding the desired target DNA sequence. A novel selection vector is used to reduce artifacts. Heterooligimeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.
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