Very rapid detection of fungal infections
US5098830A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 16, 1990 |
| Grant date | Mar 24, 1992 |
| Priority date | — |
| Expiry date | Jul 16, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2326/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the detection of a fungal infection within 1-2 hours comprises the steps of: (a) contacting a sample suspected of containing a peroxidase-containing fungus with a mixture which comprises (i) a peroxide from hydrogen peroxide and addition compounds thereof containing latent hydrogen peroxide; (ii) a scavenger to remove heavy metals which may potentially decompose said peroxide catalytically; (iii) an alkaline buffer solution having the capacity to maintain the pH at a value within the range of 9.5 to 14; and (iv) a colorless oxidizable substrate (such as tyrosine, .beta.-(3,4-dihydroxyphenyl)-.alpha.-alamine (DOPA) or caffeic acid), which on reaction with oxygen at said pH value gives a visibly colored oxidation product; and (b) observing whether a color develops indicating the presence of peroxidase-containing fungus in the sample. Also included is a test kit for carrying out the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.