Macrolide biosynthetic genes for use in streptomyces and other organisms
US5098837A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 7, 1988 |
| Grant date | Mar 24, 1992 |
| Priority date | — |
| Expiry date | Jun 7, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/886
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Spiramycin antibiotic biosynthetic genes of Streptomyces ambofaciens are provided by the present invention, in addition to a variety of recombinant DNA vectors. The genes also function in other macrolide producing organisms. The genes can be used to increase or otherwise alter the macrolide antibiotic-producing ability of an organism. The present invention also provides host strains comprising mutant spiramycin biosynthetic genes which can be used to generate novel antibiotics. Also provided is a method for preparing the mutant gene comprising mutating cloned spiramycin biosynthetic DNA by transposon mutagenesis with subsequent transformation into a macrolide-antibiotic producing host and homologous recombination into its genome, to generate stable mutant cell lines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.