Method for conducting nucleic acid hybridization in chamber with precise fluid delivery

US5100775A · kind A · utility

Inventors

• Peter J. Smyczek · Milwaukee, US
• John A. Thompson · Berkeley, US

Key dates

• Filing date: Mar 12, 1990
• Grant date: Mar 31, 1992
• Expiry date: Mar 12, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M41/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An improved method for carrying out multiple laboratory processes including hybridization, cell growth and chromatographic separations including steps of: providing a laboratory apparatus having a hollow recessed chamber and a removable cover sized to be slidably retained in at least a portion of the hollow recessed chamber for providing a variable volume chamber. Inserting a sandwich including a membrane having DNA fragments bound thereto, and a porous non-reactive filter on either side of the membrane into the chamber and inserting the slidable cover, a distance sufficient to provide a minimum volume chamber for the sandwich. Delivering a pre-hybridization solution to the chamber by utilizing a fluid administration kit in fluid communication with the chamber and pre-hybridizing the DNA fragments at a desired temperature. Removing the pre-hybridization solution from the chamber by the fluid administration kit. Delivering a probe/hybridization solution to the chamber by the fluid administration kit and hybridizing the DNA fragments at the same temperature as pre-hybridization. Removing the hybridization solution from the chamber by the fluid administration kit. Passing a low salt w…