Lipase immobilized with hydrophilized polyolefin fibers for resolving racemates

US5102799A · kind A · utility

Assignee

• BASF SE

Inventors

• Dieter Urban · Speyer, DE
• Wolfgang Ladner
• Axel Paul · Lampertheim, DE

Key dates

• Filing date: Nov 4, 1988
• Grant date: Apr 7, 1992
• Expiry date: Nov 4, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P17/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Immobilized lipase is prepared by forming a mixture of hydrophilized polyolefin fibers, crude porcine pancreas lipase, one or more water soluble dihydric to hexahydric aliphatic alcohols of 2 to 6 carbons and an aqueous buffer solution of pH 5-9 at a temperature of 0.degree. to 40.degree. C., allowing the mixture to stand for 1 minute to 3 days, filtering the mixture and washing the resultant filter cake. The dihydric to hexahydric alcohol may be combined with one or more water soluble monohydric alcohols having 1 to 4 carbon atoms. The immobilized lipase is used for resolving racemates of esters of racemic alcohols in an aqueous medium.