Quantitative determination of bilirubin and a reagent therefor
US5104794A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Mar 13, 1990 |
| Grant date | Apr 14, 1992 |
| Priority date | — |
| Expiry date | Mar 13, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/146666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a process for measuring an amount of all bilirubins in a specimen, comprising deconjugating conjugated bilirubins with a reagent comprising an enzyme capable of deconjugation to form the unconjugated bilirubin, and determining an amount of the unconjugated bilirubin in the specimen, and a reagent therefor. The present invention also provides a process for measuring an amount of the unconjugated bilirubin in a specimen, comprising oxidizing and eliminating conjugated bilirubins in the specimen with an oxidizing agent capable of oxidizing bilirubins, in the presence of a metal ion forming a complex with the bilirubins and a surfactant at pH 6.5 or less, and determining an amount of the unconjugated bilirubin in the specimen, and a reagent therefor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.