Method and apparatus for cleaving deoxyribonucleic acid
US5106585A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 29, 1984 |
| Grant date | Apr 21, 1992 |
| Priority date | — |
| Expiry date | Oct 29, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention relates to a procedure for chemically determining the base sequence of DNA and, in particular, its step of cleaving the labeled DNA having undergone a base-selective chemical modification at the position of the modified base. More particularly, it relates to a method for cleaving DNA which comprises modifying DNA base-selectively with chemical reagents, bringing the resulting reaction mixture into contact with an adsorbent to adsorb the chemically modified DNA on the adsorbent, washing the adsorbent with a washing fluid to remove any chemical modification reagents from the adsorbent, bringing the adsorbent into contact with an eluent to elute the chemically modified DNA adsorbed thereon, concentrating the resulting eluate and then heating it together with a cleavage reagent. This invention also relates to apparatus for carrying out the above-described method which comprises a reactor 1 for receiving the reaction mixture having undergone a chemical modification reaction of DNA, a transfer pump 4, an adsorber 2 containing an adsorbent, a three-way valve 5 and a concentrator 3, these units being interconnected by conduits in the order mentioned.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.