Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5106727A · kind A · utility
115Cited by
1References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 13, 1990 |
| Grant date | Apr 21, 1992 |
| Priority date | — |
| Expiry date | Jul 13, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
According to this invention, a process for substantially amplifying template nucleic acid present in a sample is described, wherein said amplification may be performed without prior knowledge of specific sequences, which process comprises apposition of random oligonucleotide primers to said template nucleic acid under conditions such that extension products of said primers are synthesized which are complementary to said template nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.