Immobilization of an isethiacyanate of a cofactor on a polymer

US5106740A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Hubert Bader · Mainz, DE
• Hans-Ullrich Hoppe · Hofheim, DE
• Michael Magerstadt · Hofheim, DE
• Merten Schlingmann · Königstein, DE
• Dieter Ulschneider · Königstein, DE
• Axel Walch · Baldham, DE

Key dates

• Filing date: Dec 6, 1990
• Grant date: Apr 21, 1992
• Expiry date: Dec 6, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N11/098
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A cofactor having an aromatic or benzylic amino group is converted into an isothiocyanate by reaction with a compound such as thiophosgene and the isothiocyanate of the cofactor is attached to a polymer which is preferably water-soluble. When the polymer has amino groups, a thiourea bridge is formed between the polymer and cofactor and a polymer having hydroxyl groups results in a thiocarbamate bridge. The polymer may be a copolymer of vinylamine or vinylmethylamine and vinylmethylacetamide, partially alkylamine-substituted .alpha., .beta.-poly-(2-hydroxyethyl)-D,L-aspartamide, polyethyleneimine or carbohydrate. The cofactor bound to a polymer and an enzyme are contained in microcapsules or in a membrane to form an enzyme reactor.