Process for preparing a maltoheptaose derivative

US5108913A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Elli Rauscher · Munich, DE
• Ulrich Neumann · Peißenberg, DE
• August W. Wahlefeld · Hohenpeißenberg, DE
• Alexander Hagen · Tutzing, DE
• Wolfgang Gruber · Bischofswerda, DE
• Joachim Ziegenhorn · Starnberg, DE
• Eugen Schaich · Weilheim, DE
• Ulfert Deneke · Fürth, DE

Key dates

• Filing date: Jun 27, 1990
• Grant date: Apr 28, 1992
• Expiry date: Jun 27, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

.alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.