Method of somatotropin naturation using urea and a soluble organic alcohol
US5109117A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 26, 1990 |
| Grant date | Apr 28, 1992 |
| Priority date | — |
| Expiry date | Jun 26, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the solubilization and naturation of somatotropin from refractile bodies produced by r-DNA technology wherein the refractile bodies are dissolved in an aqueous solution comprising a chaotropic agent such as urea or guanidine hydrochloride and a soluble organic alcohol such as isopropanol or benzyl alcohol. The solubilized protein is exposed to mild oxidation for a time sufficient to allow the protein to form disulfide bonds and refold to its native conformation. The presence of the alcohol suppresses the formation of somatotropin dimers and aggregates and results in higher yields of the desirable monomeric form of the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.