Nucleic acid probe linked to a label having a terminal cysteine
US5109124A · kind A · utility
215Cited by
10References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 2, 1989 |
| Grant date | Apr 28, 1992 |
| Priority date | — |
| Expiry date | Feb 2, 2009 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K2121/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A polynucleotide probe with a label bearing a plurality of signalling moieties. The label is attached to the probe by the reaction of an amino and sulfhydryl reactive hetero bifunctional reagent with the probe and label, the reaction resulting in the oxidation of a sulfhydryl group of the label. The label may be attached to the 5' terminus of the probe, or to modified bases of the probe. Probes constructed according to the invention are useful in detecting target sequences in genomic DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.