Purificaiton procedure of tPA from crude preparations
US5110736A · kind A · utility
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Key dates
| Filing date | Oct 24, 1990 |
| Grant date | May 5, 1992 |
| Priority date | — |
| Expiry date | Oct 24, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21069
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Crude tPA containing, as an impurity, a protein which is reactive with an anti-human tPA antibody and has a molecular weight of 110,000.+-.20,000 daltons is purified by bringing the crude tPA into close contact with an affinity reagent containing an immobilized Kunitz inhibitor which is produced in seeds of Erythrina latissima and other Erythrina plants and acts as an inhibitor on trypsin, plasmin and tPA but does not act on urokinase, so that tPA is collected selectively.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.