High performance microcapillary gel electrophoresis
US5112460A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 13, 1989 |
| Grant date | May 12, 1992 |
| Priority date | — |
| Expiry date | Oct 13, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44752
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A microcapillary column for high performance electrophoresis. A preferred column includes a microcapillary, a thin layer of coating material covalently bonded to the inner surface of the microcapillary wall, and a gel comprising polyacrylamide polymerized in the tube, filling it. The gel-containing microcapillary is prepared by covalently bonding a layer of a suitable coating material to the inner surface of the microcapillary wall, and then causing a mixture of monomer with or without crosslinking agent, initiator, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates and in some instances over 1,000,000 theoretical plates within separation times of less than thirty minutes, permits trace level determinations of molecular weights, and permits electrophoretic operation at fields of 300 V/cm or higher, resulting in extremely high resolution separations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.