Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA
US5112734A · kind A · utility
59Cited by
4References
66Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 26, 1989 |
| Grant date | May 12, 1992 |
| Priority date | — |
| Expiry date | May 26, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention pertains to an improved method for detecting a nucleic acid target sequence with a replicatable RNA reporter system. Two polymerase-mediated reactions are used to generate a target-specific gene containing a DNA sequence for a replicatable RNA. Transcription of the target-specific gene yields a replicatable RNA which is amplified by replication. Synthesis of the gene and the replicatable RNA is strictly dependent upon specific interaction with the target sequence. Consequently, the amplified signal (RNA) is target-dependent and background signal is reduced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.