Method of identification and preparation of probes for pestiviruses, oligonucleotides and probes thus obtained and a method of detection of pestiviruses
US5112753A · kind A · utility
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Key dates
| Filing date | Jun 12, 1990 |
| Grant date | May 12, 1992 |
| Priority date | — |
| Expiry date | Jun 12, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In the method of identification and preparation of general and/or specific probes for varieties of Pestivirus, a length of approximately 110 to 115 bases, towards the 5' end on the Pestivirus genome in the non-coding region corresponding to the region situated between bases 200 and 310 of the genome of the BVD Osloss virus, is amplified by means of converging primers in a polymerase chain reactions, the amplified length is sequenced and, by comparison with similar previously sequenced lengths of other varieties of Pestivirus, the homologous and/or specific oligonucleotidic sequences of the probes are identified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.