Modified oligonucleotides

US5112963A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Uwe Pieles · Schliengen, DE
• Uwe Englisch · Göttingen, DE
• Friedrich Cramer · Göttingen, DE

Key dates

• Filing date: Nov 14, 1988
• Grant date: May 12, 1992
• Expiry date: Nov 14, 2008

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61P31/12
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present invention provides modified oligonucleotides, wherein, amongst the first four bases on the 5'-end, they have the base sequence AT or TA and, bound to one of the two O.sup.- groups of the 5'-phosphate group of the first nucleotide at the 5'-end of said oligonucleotide, they contain a radical of the general formula: ##STR1## in which R.sub.4 is a spacer group and R.sub.1, R.sub.2 and R.sub.3 are hydrogen atoms or alkyl or alkoxy radicals containing up to 3 carbon atoms. These modified oligonucleotides can be used for blocking the replication and/or expression of genes which have a sequence complementary with the modified oligonucleotides.