Process for the production of permanently culturable animal and human cell lines and the use thereof

US5114847A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Herbert Jungfer · Starnberg, DE
• Heinrich Barchet · Deggendorf, DE
• Winfried Albert · Eberfing, DE

Key dates

• Filing date: Sep 25, 1990
• Grant date: May 19, 1992
• Expiry date: Sep 25, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for obtaining permanently culturable human and animal cell lines, as well as uses for these cell lines, are disclosed. The method involves fusing cells of a non-immortal cell line with cytoplasts or cytoplasma fractions of "immortal", transformed cells such as immortal myeloma cells, ascites-tumor cells or Epstein Barr Virus infected cells. Once fusion takes place the product is a permanently culturable variant of the previously normal cell line.