Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US5115102A · kind A · utility
7Cited by
1References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 21, 1989 |
| Grant date | May 19, 1992 |
| Priority date | — |
| Expiry date | Jul 21, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/65
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed at variant proteins and polypeptides having an enhanced affinity, i.e., greater binding strength, for immobilized-metal affinity resins and resides in engineering one or more specific metal-chelating amino acid sequences into a protein or polypeptide, the specific sequence depending on the metal-binding amino acids utilized and the secondary structure associated with the portion of the protein or polypeptide to include such sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.