Patent · US Expired

Reagent and method for measuring leukocytes and hemoglobin in blood

US5116539A · kind A · utility

31Cited by
10References
5Claims
0Family size

Assignee

Inventors

Key dates

Filing dateNov 3, 1988
Grant dateMay 26, 1992
Priority date
Expiry dateNov 3, 2008

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/101666
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A reagent for and method of measuring leukocytes or hemoglobin or both in blood using a reagent which is water-soluble mixture containing: PA1 (a) a polyoxyethylene-based nonionic surfactant represented by the formula: EQU R.sub.1 --R.sub.2 (CH.sub.2 CH.sub.2 O).sub.n --H PA1 where R.sub.1 is an alkyl, alkenyl or alkynyl group having 6 to 24 carbon atoms, R.sub.2 is --O--, ##STR1## or --COO--, and n is an integer of 6 to 50, and (b) a buffer to adjust the pH of the solution within the range of 3-11 is disclosed. Measurements of hemoglobin in blood samples are of extreme importance to clinical diagnosis of such diseases as leukemia and anemia. Measurements of eosinophils, which are in the category of leukocytes, are important to the diagnosis of allergic conditions.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.