Amino acid substituted 4-aminophenazones for measuring enzyme activity

US5116730A · kind A · utility

Assignee

• THE BOARD OF GOVERNORS OF WAYNE STATE UNIVERSITY · US

Inventors

• Joseph D. Artiss · Windsor, CA
• Jill M. Bensie · Ferndale, US
• Bennie Zak · Beverly Hills, US
• Daniel S. Raden · Hawthorn Woods, US

Key dates

• Filing date: Jun 15, 1988
• Grant date: May 26, 1992
• Expiry date: Jun 15, 2008

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilirubin oxidase.