Material for affinity chromatography which is a sulfated polysaccharide bonded to an insoluble polymer

US5116962A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Werner Stuber · Lahntal, DE
• Eric-Paul Paques · Marburg, DE

Key dates

• Filing date: Jul 16, 1990
• Grant date: May 26, 1992
• Expiry date: Jul 16, 2010

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2220/54
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A process for the preparation of a material for affinity chromatography is described, in which a sulfated polysaccharide is bonded to a carrier material which has amino groups. For this purpose, a sulfated polysaccharide is treated with an oxidizing agent which oxidizes glycols to aldehydes, with cleavage of the carbon chain, and this modified sulfated polysaccharide is allowed to react with a carrier which has amino groups. It is possible using the described affinity material to adsorb proteins which bind to sulfated polysaccharides from solutions of these proteins and, where appropriate, then to desorb them, for example antithrombin III from blood plasma.