Methods for detecting cellular pathology by assaying spectrin and spectrin breakdown products
US5118606A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 26, 1990 |
| Grant date | Jun 2, 1992 |
| Priority date | — |
| Expiry date | Jul 26, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method for the detection of cellular pathology by means of an immunoassay to determine the presence of stable breakdown products, termed BDP's or BDP1 and BDP2, of the cytoskeleton component spectrin. In one aspect of the invention, the components from a sample of spectrin-containing cells are physically separated, as by exposure to an electric field, in such a way that BDP and spectrin are separated. Antibodies reactive with BDP are then contacted with the separated sample, and their binding to that portion of the sample containing any BDP determined. In another aspect of the invention, an assay, such as an ELISA assay, is performed to detect total spectrin immunoreactivity as an indication of cellular death or degradation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.