DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside
US5118802A · kind A · utility
262Cited by
5References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 27, 1991 |
| Grant date | Jun 2, 1992 |
| Priority date | — |
| Expiry date | Feb 27, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.