Vector comprising DNA sequence coding for enzyme-donor polypeptide
US5120653A · kind A · utility
20Cited by
3References
4Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 22, 1985 |
| Grant date | Jun 9, 1992 |
| Priority date | — |
| Expiry date | Oct 22, 2005 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.