Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (NADH) in solution
US5122456A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 24, 1989 |
| Grant date | Jun 16, 1992 |
| Priority date | — |
| Expiry date | Feb 24, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed for the quantitative determination of 1,4-dihydronicotinamide adenisne dinucleotide (NADH) in solution. The method comprises contacting the NADH-containing solution with an activated carbon electrode, maintaining the carbon electrode at a controlled, fixed potential effective to cause oxidation of NADH at the electrode surface, and measuring the current output from the carbon electrode, wherein there is used a noble metal containing preferably a platinized or palladized activated carbon electrode comprising a porous, heterogeneous, resin-bonded layer of activated carbon or graphite particles comprising the finely divided noble metal preadsorbed thereon and bonded together with a natural or synthetic resin binder, preferably a hydrophobic resin such as polytetrafluoroethylene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.