Methods for trans-destabilization of specific proteins in vivo and DNA molecules useful therefor
US5122463A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 17, 1990 |
| Grant date | Jun 16, 1992 |
| Priority date | — |
| Expiry date | May 17, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/95
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention pertains to a method of metabolically destabilizing a protein or peptide of interest in vivo provided that the protein or peptide contains a second determinant of the N-end rule-based degradation signal and provided that another (targeting) protein or peptide can be identified that specifically interacts with the protein or peptide of interest. The methods of the invention comprise contacting the protein or peptide of interest with the targeting protein or peptide which contains a destabilizing amino-terminal amino acid according to the N-end rule of protein degradation but lacking a second determinant of the N-end rule-based degradation signal. Because nearly all proteins specifically interact with other proteins, this is a broadly applicable method for metabolically destabilizing a protein or peptide of interest in vivo.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.