Application of resins consisting of functional polymers as the stationary phase in affinity chromatography for the purification of growth factors, and corresponding purification method
US5122597A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 1988 |
| Grant date | Jun 16, 1992 |
| Priority date | — |
| Expiry date | Apr 15, 2008 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to the application, as the stationary phase in affinity chromatography for the purification of the FGF type growth factors, of the polymers or copolymers onto which --SO.sub.3 H and --SO.sub.3 M groups are randomly bound, groups in which M denotes a physiologically acceptable metal, as well as, preferably, --SO.sub.2 R groups, in which R denotes a radical obtained by removal of a hydrogen atom from the amino group of an amino acid or an amino acid derivative. The invention relates also to the obtaining of a purified bFGF or aFGF growth factor, or a mixture of these growth factors, consisting in carrying out an affinity chromatography on a resin such as defined hereinabove, by carrying out an elution with a neutral pH buffer having an ionic strength equivalent to that of a 0.15 M to 2.5 M NaCl solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.