Process for sequencing nucleic acids

US5124247A · kind A · utility

Assignee

• Europaeisches Laboratorium Fuer Molekularbiologie (EMBL) · DE

Inventor

• Wilhelm Ansorge · Gaiberg, DE

Key dates

• Filing date: Dec 11, 1989
• Grant date: Jun 23, 1992
• Expiry date: Dec 11, 2009

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

For the sequencing of nucleic acids by enzymatic extension of an oligonucleotide primer in the presence of a polymerase, the four deoxyribonucleoside triphosphates, one of the four dideoxynucleoside triphosphates and the nucleic acid to be sequenced as template in each of four different preparations, labelling of the nucleic acid fragments which form and which are dependent on the dideoxynucleoside triphosphate used, separation by gel electrophoresis of the preparations containing the fragments and detection of the sequence via the label, at least two of the dideoxynucleoside triphosphates are used in different amounts together in one preparation and the fragments are differentiated by the intensity of the labelling signal of the bands in the gel or in another separation procedure.