Process for detecting polymorphisms on the basis of nucleotide differences
US5126239A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 1990 |
| Grant date | Jun 30, 1992 |
| Priority date | — |
| Expiry date | Mar 14, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.