Utilization of plant protoplasts
US5126263A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 24, 1990 |
| Grant date | Jun 30, 1992 |
| Priority date | — |
| Expiry date | May 24, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/878
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Plant protoplasts are exposed for interaction with an external agent by exposing the plasma membrane of protoplasts in the root hairs of the plant without release of protoplasts from the plant. This may be achieved by enzymatically degrading the plant cell wall at the apices of root hairs under conditions which expose the plasma membrane whilst maintaining or readily permitting restoration of the essential functional integrity of the plant. Enzymatic degradation of the cell wall of the apices of root hairs is possible in a wide range of crop species and other species and to expose the plasma membrane with partial protoplast release. This enables direct interaction with plasmids, viruses and micro-organisms at the surface of the plasma membrane of their exposed protoplasts, or the fusion of these with isolated protoplasts of other plant systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.