Analytical method using tetrazolium salt indicators having a reflectance plateau

US5126275A · kind A · utility

Assignee

• Miles Inc. · US

Inventors

• Robert P. Hatch · Elkhart, US
• Jürgen Köcher · Cologne, DE
• Nan-Horng Lin · Mundelein, US
• Scott Ruetten · Granger, US
• Klaus Wehling · Wuppertal, DE

Key dates

• Filing date: Sep 19, 1990
• Grant date: Jun 30, 1992
• Expiry date: Sep 19, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

General classes of 2-thiazolyl tetrazolium salt compounds have been found to be characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic indicators for reducing substances such as NADH. The reflectance plateau confers improved accuracy to analytical assays, particularly for the determination of analytes of medical diagnostic significance, in which a colorimetric response on a reagent carrier matrix is measured by reflectance.