High performance Zimm chromatography--HPZC
US5129723A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 1991 |
| Grant date | Jul 14, 1992 |
| Priority date | — |
| Expiry date | Apr 11, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/4711
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed based on a single injection into a modified HPLC line by which means the light scattering data required to characterize the injected molecular suspension in terms of its weight-average molecular weight, z-average square radius, and second virial coefficient may be measured and collected. The method requires that a molecular suspension at a fixed concentration be prepared and injected into a flowing stream of pure solvent following standard liquid chromatographic procedures. The sample is then passed directly to a mixing chamber whose volume is preferably several times that of the injected volume. After this mixing, the sample enters a light scattering detector where its absolute scattering intensity as a function of angle is measured for each eluting fraction. From the data so-recorded, the concentration of each fraction may be calculated and a Zimm plot may be made to yield the required molecular parameters.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.