Method for determining the .beta.-subunit of human prolyl hydroxylase by radioimmunoassay to detect hepatic disease
US5130233A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 28, 1989 |
| Grant date | Jul 14, 1992 |
| Priority date | — |
| Expiry date | Dec 28, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining human prolyl hydroxylase by radioimmunoassay according to the sandwich method wherein a monoclonal antibody to human prolyl hydroxylase and polyclonal antibody to human prolyl hydroxylase are used, characterized in that a monoclonal antibody to human prolyl hydroxylase is used as at least one of the antibodies which are to be coated on a solid support and to be labeled with a radioactive element. This method is simple and operable with small amounts of samples and gives exact results. Thus, this method is useful for the diagnosis of hepatic diseases. A monoclonal antibody specific to the .beta.-subunit of human prolyl hydroxylase is used to test for the human prolyl hydroxylase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.