Method of quantitative determination utilizing enzyme aggregation
US5130234A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 1989 |
| Grant date | Jul 14, 1992 |
| Priority date | — |
| Expiry date | Aug 28, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/819
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A quantitative determination of a substance is performed in a homogeneous system based on a change in enzyme activity; differences between the activity of a free enzyme and that of an enzyme bound by an aggregation or chemical bonding are observed. A peroxidase-labeled antibody and antigen system is one of of the typical example. Since the reaction is effected in a homogeneous system, the amount of antigen can be easily measured by the difference of enzyme activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.