Patent · US Expired

Method of quantitative determination utilizing enzyme aggregation

US5130234A · kind A · utility

2Cited by
10References
6Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 28, 1989
Grant dateJul 14, 1992
Priority date
Expiry dateAug 28, 2009

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S436/819
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A quantitative determination of a substance is performed in a homogeneous system based on a change in enzyme activity; differences between the activity of a free enzyme and that of an enzyme bound by an aggregation or chemical bonding are observed. A peroxidase-labeled antibody and antigen system is one of of the typical example. Since the reaction is effected in a homogeneous system, the amount of antigen can be easily measured by the difference of enzyme activity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.