Method for cryopreserving musculoskeletal tissues
US5131850A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 3, 1989 |
| Grant date | Jul 21, 1992 |
| Priority date | — |
| Expiry date | Nov 3, 2009 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/125
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
Disclosed herein is a method for cryopreserving musculoskeletal tissues, such as ligaments, tendons and cartilage, by placing such tissue in contact with a composition containing a cryopreserving agent comprising a cell-penetrating organic solute, which is preferably dimethylsulfoxide, and a glycosaminoglycan, which is preferably chondroitin sulphate, in an amount sufficient to cryopreserve the musculoskeletal tissue. The addition of a glycosaminoglycan to a cryopreserving agent comprising a cell-penetrating organic solute permits a broad range of cooling rates to be employed, rather than the very narrow ranges which are employed using a cryopreserving agent comprising a cell-penetrating organic solvent without the glycosaminoglycan. Also disclosed are a freezing schedule designed to maximize retention of tissue cell viability and biomechanical properties during and after the freezing process, and a thawing schedule which maximizes cell viability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.