Accelerated nucleic acid reassociation method
US5132207A · kind A · utility
22Cited by
1References
44Claims
0Family size
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Key dates
| Filing date | Jan 23, 1991 |
| Grant date | Jul 21, 1992 |
| Priority date | — |
| Expiry date | Jan 23, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid molecules in a single phase reaction solution is disclosed wherein the rate of reaction is greatly increased over the rate of reaction at standard reference conditions. The greatly accelerated reaction rate is accomplished through the use of known concentrations of nucleic acid precipitating agents which are added to the reaction solution. Nucleic acid denaturing agents may also be added. The solution so formed is incubated and then assayed for the presence of double stranded nucleic acid molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.