Process and medium for cloning and long-term serial cultivation of adult human endothelial cells
US5132223A · kind A · utility
19Cited by
1References
11Claims
0Family size
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Key dates
| Filing date | Nov 5, 1990 |
| Grant date | Jul 21, 1992 |
| Priority date | — |
| Expiry date | Nov 5, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/91
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Vigorous growth of endothelial cells from human blood vessels in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.