Device for the detection of selected motile organisms
US5132229A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 23, 1990 |
| Grant date | Jul 21, 1992 |
| Priority date | — |
| Expiry date | Apr 23, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A device for detecting the presence of a particular motile organism within a sample are disclosed. The sample may be derived from dry milk, raw meat, poultry or a clinical specimen. A preferred method of using the device includes inoculating a selective enrichment medium containing a chemotactic attractant with the sample and contacting the selective enrichment medium with a nonselective motility medium containing a chemotactic attractant in a concentration less than the attractant concentration in the selective enrichment medium. Upon incubation, the motile organism metabolizes the chemotactic attractant, allowing the organism to move into the motility medium where it interacts with antibodies specific for the organism, thereby causing the formation of a persistent immobilization band. The device is particularly useful in detecting Salmonella. This application is a divisional of U.S. Ser. No. 773,201, filed Sep. 9, 1985 and issued as U.S. Pat. NO. 4,920,063 on Apr. 24, 1990, which application is a continuation-in-part of U.S. Ser. No. 621,182, filed Jun. 15, 1985 and issued as U.S. Pat. No. 4,563,418 on Jan. 7, 1986, which application is a continuation of Ser. No. 366,978 filed Ap…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.