Type II restriction endonuclease SgrAI

US5134069A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Klaus Kaluza · Bad Heilbrunn, DE
• Bruno Frey · Penzberg, DE
• Gudrun Schmitz-Agheguian · Bernried, DE
• Michael Jarsch · Bad Heilbrunn, DE
• Christoph Kessler · Munich, DE

Key dates

• Filing date: Nov 16, 1990
• Grant date: Jul 28, 1992
• Expiry date: Nov 16, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/897
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.